Improved method for direct counting of basophil leucocytes.

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Improved method for direct counting of basophil leucocytes.

An improved method for the direct counting of basophil leucocytes from venous or capillary blood is described. The method is based upon the use of cetylpyridinium chloride to lyse the erythrocytes and render the muco-polysaccharides of the granule insoluble. Aluminium sulphate is used to mordant toluidine blue to the granules. Our knowledge of the role of the basophil leucocytes in disease has ...

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A multi-step centrifuge method for extraction of leucocytes and preparation of Tc-HMPAO labeled leucocytes

Background: Labeled leucocytes could be used for localization of infection foci after surgeries or in inflammatory diseases including inflammatory bowel diseases. Extraction of leucocytes needs 10% Hetastarch which is not available in Iran. We provide a method employing multiple centrifuges to extract and label leucocytes with Tc-HMPAO. Methods: The study was conducted from April to June 2018 ...

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Basophil counting with a new staining method using alcian blue.

Difficulties in obtaining reproducible and accurate enumeration of circulating basophils with existing techniques have hampered investigation of this infrequent cell population. A new basophil staining method is described that employs alcian blue dye for staining of heparin within basophils at low pH and in the presence of lanthanum ions. Basophil recognition is facilitated by reducing nonspeci...

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An improved box-counting method for image fractal dimension estimation

Article history: Received 6 September 2007 Received in revised form 14 January 2009 Accepted 2 March 2009

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Improved Algorithms of Direct Torque Control Method

In the paper a new way of DTC control method analysis to explain flux and current distortion at a low speed of motor operation is discussed. As a result, a new DTC-δ algorithm is proposed. It is important that the DTC-δ algorithm uses the same switching table as the DTC method. The modified DTC algorithms are free from the disadvantages of the conventional DTC method i.e. hexagonal flux and str...

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 1966

ISSN: 0021-9746

DOI: 10.1136/jcp.19.4.402